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Figure 5
Southern blots of various organisms’ DNA using a radioactive probe from the Antennapedia gene of Drosophila melanogaster. Because we do not expect the sequences between such diverged species to be perfectly identical, the stringency of the hybridization is lowered by changing the salt conditions. (Such low-stringency blots across phyla are colloquially refered to as "zoo blots," for obvious reasons.) Autoradiography shows that Drosophila genes contain several portions that are like Antennapedia genes in structure and that many organisms contain several genes that will hybridize this radioactive gene fragment, suggesting that Antennapedia-like genes exist in these organisms. The numbers beside the blots indicate size of bands, in kilobases. (From McGinnis et al., 1984, courtesy of W. McGinnis.)
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